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Echocardiographic assessments were performed with sedation using 50 mg/kg of intraperitoneal ketamine and 5 mg/kg of xylazine, ensuring stable positioning

Ratiometric time-course measurements were carried out with initially oxidized or reduced roGFP2 on a BMG POLARstar Omega fluorescence plate reader with a filter-based excitation at 39010 and 48010 nm

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While it is unlikely to get too much of a micronutrient if you are taking a supplement, getting too much, known as vitamin toxicity, is possible